Determination of Photosynthesis-Related and Ascorbate Peroxidase Gene Expression in the Green Algae (Chlorella vulgaris) Under High-Temperature Conditions

Increasing water temperatures because of climate change resulted in population shifts and physiological responses aquatic environments. In this study, short-term high-temperature condition effects on green algae Chlorella vulgaris were investigated at transcriptional levels. The photosystem II D1 protein (psbA) gene, a large unit Rubisco (rbcL) gene chloroplastic ascorbate peroxidase (cAPX) expressions quantified using semi-quantitative real time-PCR. psbA transcription level 45˚C for 48 72 h was reduced by approx. 2.22 2.86-folds, respectively. rbcL also 1.54 relative to the control h. Our APX results indicated that significantly increased 35˚C 24, 48, contrast, cAPX mRNA transcript 2 times compared with control. data demonstrated alteration expression could play an essential role acclimation C. vulgaris.